077 In Vitro Growth of Immature Peach [Prunus persica (L.) Batsch] Embryos Related to Carbohydrate Source

in HortScience
View More View Less
  • 1 Dept. of Horticultural Sciences, Texas A&M Univ., College Station, TX 77843-2133

Carbohydrate energy source of various tissue culture media has an effect on growth and survival of the explants. Sucrose is the standard carbohydrate used in most tissue culture systems. The objective of the study was to determine the effect of five carbohydrate sources (fructose, glucose, maltose, sorbitol, and sucrose) at two levels (2% and 3%) on germination, growth, and survival of immature peach embryos (9.7 to 14.7mm) in vitro. Five cultivars were used. Overall, fructose, maltose, and sucrose each stimulated germination and growth as the primary carbohydrate energy source of peach embryo culture to the same degree; glucose and sorbitol were inferior. However, fructose was superior to sucrose in one cultivar. In general, sugar level did not affect survival, although cultivars did vary somewhat. Survival was found to be highly dependent upon embryo maturity.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 56 12 1
PDF Downloads 84 34 0