070 Genetic Identification of Oncidium Varieties by RAPD Markers

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  • 1National Pingtung University of Science & Technology, Pingtung, Taiwan; 2Taiwan Flower Biotechnology Co., Taiwan; 3Institute of Botany, Academia Sinica, Taiwan

Molecular markers by random amplified polymorphic DNAs were used to evaluate the genetic variation among different Oncidium accessions. It is possible to distinguish different registered Oncidium hybrids, including Gower Ramsey, Sweet Sugar, and Taka using nine random primers. Furthermore, variation was also detected within different cultivars derived from same hybrids. For example, several cultivars of Gower Ramsey could be distinguished based on molecular markers. Based on dendrogram, the investigated cultivars were clustered into several groups. Onc. Gower Ramsey and its selected cultivars were in one group. Onc. Sweet Sugar, Onc. Taka and Onc. Sharry Baby `Sweet Fragrance' were clustered in separate groups.

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