The grape Vitis champinii Planchon is one source of nematode resistance in grape rootstocks. Several selections valued for their resistance to the root-knot nematode (Meloidogyne incognita), a serious pest of grape production, are used as rootstocks and in rootstock variety development. However, V. champinii-based rootstock varieties are faulted for their excess vigor and susceptibility to other root pests. Root-knot nematode populations with the ability to damage important V. champinii-based rootstocks have been identified and may become more common. Other V. champinii accessions might be sources of nematode resistance genes with different specificities or might have more suitable horticultural characteristics than V. champinii varieties in commercial use. Nine V. champinii accessions from the National Clonal Germplasm Repository, Davis, Calif., and a V. champinii rootstock variety were screened for resistance to M. incognita. Resistance was assessed by counting eggs produced per root system. Eight of ten V. champinii accessions did not support nematode reproduction. Susceptible accessions supported lower nematode reproduction than susceptible V. vinifera control varieties. Progeny testing from crosses of resistant and susceptible accessions suggests that a dominant and a recessive gene may condition root-knot nematode resistance.