Acerola (Malpighia glabra L.) is a small, red fruit that is native to the West Indies, but is also grown in South and Central America. In western Mexico, this crop is very important because acerola is the richest known natural source of vitamin C, with a content of 1000 to 4500 mg/100 g of fruit. In nursery and field conditions, acerola growth is severely affected by root-knot nematode. The objective of this study was to evaluate the use of commercial formulations of Bacillus spp. on root-knot nematode management. This study was carried out in the Farm Santa Clara Maria in Colima State. Acerola plants, 60 days old were used. They were grown in 3-L pots with soil, compost, and pumice stone mixture as substrate. Treatments evaluated were: 5, 10, 15 and 30 mL/pot of Activate 2001, Tri-Mat (5 mL/pot) and control, without application. Activate 2001® is a concentrated liquid in water suspension of Bacillus chitinosporus, B. laterosporus, and B. licheniformis. Initial nematode population was of 3,305 in 50 g of roots. Acerola plants were harvested at 30, 60, and 90 days after application. Results show that Activate 2001 at 10 and 30 mL rates reduce significantly root-knot populations in acerola plants 60 days after application with 135 and 178 nematodes/50 g of roots, respectively. Diameter stem, shoot fresh and dry weight and root production were also increased by rhizobacteria application. These results are promising and confirmed the potential of Bacillus as a biological agent for nematode management.