Seedlings of Catharanthus roseus (L.) G. Don `Pacifica Red' were transplanted into substrates composed of either 80% sphagnum peat or coir with the remaining volume being perlite, sand, or vermiculite. The six substrates were inoculated with Pythium irregulare Buisman at 0 or 50,000 oospores per 10-cm container. The containers were irrigated daily to maintain moisture levels near container capacity. No visually apparent symptoms of infection or significant differences in shoot and root fresh and dry weights were observed among the uninoculated substrates and the inoculated coir substrates. Inoculated peat substrates had an 80% infection rate and significantly reduced shoot and root fresh and dry weights as compared to uninoculated substrates. Seedlings of C. roseus were transplanted into pasteurized and unpasteurized substrates composed of 80% (v/v) coir or sphagnum peat with the remaining 20% being perlite. Substrates were inoculated with 0, 5000, or 20,000 oospores of P. irregulare per 10-cm container. No visually apparent symptoms of infection or significant differences in shoot and root fresh and dry weights were observed among the uninoculated substrates and the inoculated pasteurized coir. The inoculated pasteurized peat substrate, inoculated unpasteurized peat substrate, and the inoculated unpasteurized coir substrate grown plants had an 88% infection and a significant reduction in the shoot and root fresh and dry weights.
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