Optimization of Media Constituents for Shoot Regeneration from Leaf Callus Cultures of Decalepis hamiltonii Wight. & Arn.

in HortScience
Authors:
Jacob GeorgePlant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore 570 013, India

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Harsh Pal BaisPlant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore 570 013, India

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G.A. RavishankarPlant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore 570 013, India

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P. ManilalPlanning, Monitoring and Coordination Department, Central Food Technological Research Institute, Mysore 570 013, India

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Response surface methodology was utilized in statistical optimization of three quality factors (the number of multiple shoots, shoot length, and number of leaves) pertaining to regeneration of plantlets from leaf calli of Decalepis hamiltonii Wight. & Arn. (swallow root). The variables evaluated were the levels of sucrose, BA, and NAA each at two different concentrations. Response surfaces for shoot length and multiple shoot number were useful in achieving optimal levels of media constituents and in understanding their interactions, but response surfaces for number of leaves were not. The data indicate that sucrose, BA, and NAA levels may be manipulated to increase or decrease quality factors chosen. This approach may be useful in developing a micropropagation protocol for D. hamiltonii. Chemical names used: benzyladenine (BA); napthaleneacetic acid (NAA).

Contributor Notes

To whom reprint requests should be addressed. Fax: +91-821-517233; E-mail: pcbt@cscftri.ren.nic.in
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