A broccoli (Brassica oleracea var. botrytis L.) seedling bioassay was used to measure paclobutrazol activity and distribution in two growing media following drench or subirrigation applications. The bioassay exhibited a saturation-type response curve for paclobutrazol concentrations up to 1000 μg·L-1 in solution and 100 μg·L-1 in the media. The concentration of paclobutrazol required to achieve one-half of the maximum observed bioassay activity was 3-fold as high in bark-based commercial potting medium as in a peat-based medium. Less than 2% of applied paclobutrazol leached out during the drench application despite the collection of up to 50 mL of leachate per 120 mL of the solution (1000 μg·L-1) that was applied per 15-cm pot. Immediately following drench application, paclobutrazol concentrations in both media were highest in the uppermost 2.5 cm and decreased downward. By 3 weeks after treatment, drench-applied paclobutrazol had moved into lower depths. Distribution of paclobutrazol was limited to the bottom 2.5 cm of media when applied as a subirrigation soak. Chemical name used: (±)-(R*,R*)-β-[(4-chlorophenyl)methyl]-α-(1,1-dimethyl)-1H-1,2,4-triazole-1-ethanol (paclobutrazol).