The objective of this study was to determine the effect of micronutrient fertilization on seedling growth in pine bark with pH ranging from 4.0 to 5.5. Koelreuteria paniculata (Laxm.) was container-grown from seed in pine bark amended (preplant) with 0, 1.2, 2.4, or 3.6 kg/m3 dolomitic limestone and 0 or 0.9 kg/m3 sulfate-based micronutrient fertilizer (Micromax ®). Initial pine bark pH for each lime rate was 4.0, 4.5, 5.0, and 5.5, respectively. Final pH (week 10) ranged from 4.7 to 6.4. Ca and Mg supply in irrigation water was 10.2 and 4.2 mg·L–1. Seedlings were harvested 10 weeks after planting, and shoot dry weight and height were determined. Pine bark solution was extracted using the pour-through method at 3, 7, and 10 weeks after planting. Solution pH was measured, and solutions were analyzed for Ca, Mg, Fe, Mn, Cu, and Zn. Shoot dry weight and height were higher in micronutrient-amended bark than in bark without added micronutrients. Lime (1.2 kg·