562 Plant Regeneration from Embryo and Cotyledons of Laiyang Dwarf Cherry

in HortScience

Laiyang dwarf cherry (Prunus pseudocerasus Lindl. Var. Laiyang dwary cherry) is an important dwarf type of Chinese cherry. It is both directly used for production and used as rootstock of sweet cherry (P. avium L.). Material used in this study was Laiyang dwarf cherry seed, which is open-pollinated. The fruit surface is sterilized with 70% ethanol on sterile working table, the seed is removed from fruit, and embryo is taken out of cracking seed shell. Explants include whole embryo, embryo without cotyledons, and cotyledons. Basal media are MS and 1/4MS major elements, sugar 30 g·L–1, agar 5.5 g·L–1, adjusted pH 5.8. Hormone used included 6-BA,IBA and 2,4-D for a total eight treatments. Culture temperature was 25° ±2°C, and photoperiod was 14 h. Results show 1) the plant regeneration rate from cotyledons is highest, second is from embryo without cotyledons, lowest is from whole embryo; 2) when BA is in the range of 0.7 to 5 ppm, plant regeneration is higher at a high concentration than that at low concentration; 3) at the same concentration, plant regeneration rate on the medium supplemented with 2,4-D is higher than that on the medium supplemented with IBA; 4) plant regeneration rate is higher on the medium containing cytokinin and auxin is higher than that on the medium only containing cytokinin; and 5) plant regeneration rate of continuous light culture cotyledons is higher than that of first dark culture 2 weeks cotyledons. Effects of light and dark/hormone and explant type on plant regeneration rate is discussed.

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