560 Molecular Analysis of the First Intron of the Chalcone Synthase A Gene in Petunia

in HortScience
Authors: R.J. Griesbach1 and R. Beck1
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  • 1 Floral and Nursery Plants Research, US National Arboretum, USDA, ARS, Beltsville, MD 20705-2350

A new method was developed to analyze genetic diversity among Petunia species. The first intron of the chalcone synthase A gene was cloned through the polymerase chain reaction (PCR) and partially sequenced. This sequence was used to dissect the intron into two halves (3' and 5' halves). The PCR primers for the 5' half amplified a single fragment that was the same length for all of the species that were studied. Restriction fragment length polymorphism (RFLP) analysis of the 5' half resulted in the same number and length of fragments for all the species that were evaluated. The PCR primers for the 3' half amplified a number of fragments that were characteristic for each species. This research provides a new tool for measuring genetic diversity. Genetic diversity measured with this tool should be closely related to evolutionary distance.

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