028 Selecting Sweetpotato Genotypes Tolerant to Chilling Stress

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  • 1 Plant and Soil Sciences Department, Mississippi State University, Mississippi State, MS 39762

A procedure to determined selection of sweetpotato (Ipomoea batatas L. Lam.) genotypes tolerant to chilling injury was initiated by crossing two resistance lines and two susceptible lines. Experimental design on the F1 progeny was a completely randomized design (CRD) with two groups, tolerant and sensitive genotypes, and 90 lines in each group. Four plants per lines were selected and each plant represented a replication. The rating of plants according to the degree of chilling injury was recorded at 36 h after chilling temperature of 5 °C with 85% relative humidity. Significance of the analysis was based on the number of plants tolerant to chilling injury from both the resistant and the sensitive groups. Results from the statistical analysis based on visual rating of the F1 progeny plants for 36 h, indicated that higher populations of resistant plants could be produced when two resistant lines were crossed in a control pollination process. Evaluations to be continued are chlorophyll fluorescence, leakage of cell content, structural changes of the cell, and peroxidase content, before and after chilling stress. These assays will be used to further determine the similarities among the chilling-tolerant genotypes. Findings will aid in elucidating mechanism of chilling injury in fruit and vegetables.

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