Manual removal of inflorescences from mature (3- and 4-year-old) American ginseng plants (Panax quinquefolium L.) at commercial timing (early July, ≈25% flowers open) increased root yield at harvest. Consecutive inflorescence removal for 2 years (third and fourth) increased yield 55.6%. Inflorescence removal in 4-year-old plants increased yield by 34.7% compared with 26.1% in 3-year-old plants. Analysis showed that the largest portion of roots (≈40%) was in the medium category (10-20 g), and inflorescence removal did not influence root size distribution. Root yield for 3-year-old plants increased quadratically with plant density, with plants lacking inflorescences having an estimated yield increase of 25%. Maximum yields of 2.4 kg·m-2 for deflowered plants were achieved at a plant density of 170 plants/m2. To maximize ginseng root yield, all plants except those needed to provide seed for future plantings should have inflorescences removed.