Greenhouse and field studies were conducted to determine the genetic relationship between bentazon tolerance exhibited by the pepper (Capsicum annuum L.) cultivars Bohemian Chili and Santaka, and to evaluate the importance of cytoplasmic factors in expression of the tolerance in `Bohemian Chili.' Greenhouse evaluation of parental and F2 populations of the cross `Santaka' × `Bohemian Chili' indicated that the major dominant gene conditioning bentazon tolerance in `Bohemian Chili' is probably the Bzt gene that conditions bentazon tolerance in `Santaka' or a gene closely linked to the Bzt locus. Field evaluation of F1 and F2 progeny populations of the cross `Bohemian Chili' × `Sweet Banana' in both `Bohemian Chili' and `Sweet Banana' cytoplasms demonstrated that cytoplasmic factors do not affect the expression of the bentazon tolerance gene in `Bohemian Chili.' We conclude that `Santaka' and `Bohemian Chili' are equally satisfactory sources of a bentazon tolerance gene for use in pepper breeding programs. Chemical name used: 3-(1-methylethyl)-(1H)-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide (bentazon).