An Efficient Method for Rooting and Acclimation of Micropropagated Apple Cultivars

in HortScience
Authors:
Jyothi Prakash BolarDepartment of Plant Pathology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY 14456

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John L. NorelliDepartment of Plant Pathology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY 14456

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Herb S. AldwinckleDepartment of Plant Pathology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY 14456

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Viola HankeInstitute for Fruit Breeding, Federal Center For Breeding Research in Cultivated Plants, Pillnitzer Platz 2, 01326 Dresden, Germany

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To root tissue-cultured apple cultivars, shoots from proliferating cultures were first transferred to root induction medium with IBA for 1 week in the dark. Shoots were later transferred to the same medium without IBA and incubated under light for elongation of the roots. Rooted shoots were then transferred to Jiffy-7s supplemented with biological plant protectant and fertilizer, and incubated in plastic humidity trays. After 2 to 3 weeks, plants were transferred to pots and covered with plastic bags to facilitate acclimation. This technique has resulted in 70% to 100% of shoots selected in vitro producing vigorously growing, healthy plants in the greenhouse. Chemical name used: indolebutyric acid (IBA).

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