Development of Sequence-specific Primers that Amplify a 1.5-kb DNA Marker for Race 1 Fusarium Wilt Resistance in Cucumis melo L.

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  • 1 Department of Plant Pathology and Physiology, Clemson University, Clemson, SC 29634
  • | 2 U.S. Department of Agriculture, Agricultural Research Service, U.S. Vegetable Laboratory, Charleston, SC 29407

Two 24-mer primers, MUSKFOM I and MUSKFOM II, were developed that amplify a 1.5-kb DNA fragment in race 1 Fusarium wilt resistant muskmelon (Cucumis melo L.), but not in race 1 susceptible germplasm tested. Three race 1 resistant cultivars and two race 1 resistant breeding lines as well as eight race 1 susceptible lines were analyzed using the two sequence-specific primers in the polymerase chain reaction. These primers should prove valuable for nondestructive determination of Fom 2 gene introgression in breeding programs.

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