Extracting DNA from Cambium Tissue for Analysis of Grape Rootstocks

in HortScience

The DNA extracted from cambium tissues of grape (Vitis spp., Muscadinia rotundifolia Small) rootstocks was found to be suitable for molecular analysis. Its quality was equivalent to that of DNA extracted from leaf tissues, although the yield was higher from leaves. The use of cambium tissue allows DNA extractions during dormancy or from grafted rootstocks where leaves are not available. The DNA extracted was suitable for restriction enzyme digestion and for analysis by restriction fragment length polymorphism (RFLP), randomly amplified polymorphic DNA (RAPD), and simple sequence repeats.

If the inline PDF is not rendering correctly, you can download the PDF file here.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 89 53 4
PDF Downloads 108 73 6