RAPD Marker Polymorphism among Saskatoon Cultivars, Clones, and Seedlings

in HortScience

Randomly amplified polymorphic DNA (RAPD) markers were used to distinguish among 16 cultivars of saskatoon (Amelanchier spp.). Eight 9-base, oligonucleotide primers amplified a total of 98 DNA fragments, of which 29 were useful as reproducible polymorphic markers. Twelve cultivars and two pairs of cultivars were uniquely characterized by these 29 markers. Polymorphism was not detected among five sources of the cv. Thiessen, whereas variability was found among seedlings from self-pollinated `Thiessen'. Samples of the cvs. Regent and Parkhill were indistinguishable from one of two sources, suggesting that the cultivars were mislabelled.

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