Phosphine (PH3) is a potential alternative fumigant to methyl bromide for insect disinfestation of cut flowers. King protea (Protea cynaroides L.), tulip (Tulipa gesneriana `Apeldoorn'), kangaroo paw (Anigozanthos manglesii Hook.), and geraldton wax (Chamelaucium uncinatum `Purple Pride') were fumigated with PH3 at varying concentrations (100 to 8000 μL·L-1) for 2, 4, or 6 hours. Vase life was evaluated at 20 °C, 65% relative humidity, and constant illumination with a photosynthetically active radiation of 15 μmol·m-2·S-1. No significant change in vase life was observed for kangaroo paws after any of the PH3 fumigations. A 6-hour fumigation at 8000 μL·L-1 significantly reduced vase life in king protea, tulip, and geraldton wax flower. Geraldton wax flower and tulip were relatively sensitive to PH3, as they were damaged by 4000 μL·L-1 for 6 hours and 8000 μL·L-1 for 4 hours, respectively. Phosphine has potential as an insect disinfestation fumigant for king protea, tulip, and kangaroo paw at 4000 (μL·L-1 for 6 hours without affecting vase life or causing damage.