Bacterial blight, incited by Xanthomonas axonopodis pv. vignicola, is a major disease of cowpea. Strong resistance has not been reported in commercial cultivars. Many cultivars released within the last ten years have not been previously screened. Thirty-eight cowpea cultivars, 60 breeding lines, and 25 PIs were screened for reaction to bacterial blight in tests conducted in a greenhouse. Inoculum was adjusted turbidimetrically (OD620nm = 0.1) to approximately 108 cfu/ml. Seedling plants were inoculated by either leaf infiltration or stem puncture methods. Ratings were on a 1-6 scale in which 1 = no symptoms, 2 = localized lesions at site of inoculation, 3 = lesions spreading slightly near site of inoculation, 4 = any symptoms on systemically infected leaves or stems, 5 = extensive wilting and/or stem collapse, 6 = dead plant. All cultivars were susceptible to the pathogen. Cream-type cultivars Bettergreen, Tender Cream, Zipper Cream, Carolina Cream, and Mississippi Cream were among the most susceptible. Breeding lines MN13, MN150, TX57069-11, TX 58048-2000, and TX 59069-11 produced hypersensitive reactions in response to leaf infiltration inoculation. However, the three TX lines were rated susceptible when inoculated by stem puncture. Eighteen PIs (including PI293467, PI293521, PI293525, PI293567, and PI293571) were highly resistant to bacterial blight.