Our understanding of the quantities and seasonal patterns of nutrient uptake by mature fruit trees has been limited by the difficulties in working with the large woody biomass of these organisms, tree-to-tree variability, and the resolution to distinguish between recently acquired nutrient from the nutrient background of the tree. We have coupled the use of stable isotopes of nitrogen (N) with periodic whole-tree excavations and nutrient analyses during the year. Vegetative growth, reproductive growth, and nutrient storage in perennial tree parts during tree quiescence represent nutrient sinks. Data obtained using mature pistachio, prune, and walnut trees indicate that macronutrient accumulation in metabolic sinks is associated with increases in tree macronutrient uptake. These data are consistent with the concept that sink removal of phloem-mobile nutrients from vascular circulation may provide the stimulus to further uptake of the nutrient(s) sequestered. We propose that the recognition of those patterns can be used to increase the efficiency of tree nutrient recovery and utilization.