Transplant studies were conducted on Taxodium distichum L., Platanus occidentalis L., Quercus shumardii Buckl., Fraxinus velutina Torr., and Chilopsis linearis (Cav.) Sweet seedlings grown in 2.2- to 9.1-L black plastic containers. Effects of half-sib family selection on post-transplant root regeneration potential (RRP) and field establishment were investigated with P. occidentalis. Taxodium distichum, Q. shumardii, and P. occidentalis were used to determine seasonal variation in relationships among RRP characteristics and measures of successful transplant establishment. Post-transplant effects of avoidance of circling root development vs. remediation practices were investigated with Q. shumardii. Effects of container media composition on field establishment and RRP of container-grown plants were studied using F. velutina and C. linearis. Impacts of rotation time on RRP and field establishment were investigated with T. distichum. Rates of RRP were the measure most consistently linked to improved post-transplant shoot growth of P. occidentalis. Utilization of locally adapted genotypes and avoidance of summer transplant were important in establishment of P. occidentalis and T. distichum. Increased small diameter root regeneration was linked to reduced water stress during transplanting of Q. shumardii. Physical characteristics of the container media impacted initial post-transplant growth of F. velutina and C. linearis.