Twenty field experiments were conducted to evaluate the response of iceberg lettuce (Lactuca sativa L.) to N and evaluate various diagnostic technologies as tools for assessing the N nutritional status of lettuce. Lettuce yields showed a curvilinear response to N in most experiments. Generally, the dry midrib nitrate-N test and the sap nitrate-N test appear to be sensitive indicators of the N nutritional status of lettuce after the folding stage of growth. The chlorophyll meter was not a sensitive indicator of the N nutritional status of lettuce. Preliminary data also show that canopy reflectance, including digital analysis of aerial photographs, is correlated to N nutritional status of lettuce. However, reflectance technologies do not readily distinguish between N deficiencies and other factors (insects, diseases, water stress, etc.) that affect plant biomass and color. Because plant tests do not appear to be sensitive indicators of N nutrition during early growth stages (before folding), a post-thinning (and pre-sidedress) soil nitrate-N test is currently being evaluated.