Seedling plugs of `Early Girl' tomato plants (Lycopersicon esculentum Mill.) were potted in peatmoss and perlite (60:40% by volume) medium, fertilized with 8, 16, 24, or 32 g NutriCote Total controlled-release fertilizer (type 100, 13N–5.67P–10.79K plus micronutrients) per pot (2.81 l), and treated with 0%, 2.5%, 5%, or 7.5% antitranspirant GLK-8924 solution, at the four true-leaf stage. Plants were tipped at the second inflorescence and laterals were removed upon emergence. Leaf stomatal conductance, transpiration rate, and growth were depressed by GLK-8924. In contrast, higher fertilization rate increased plant growth but leaf stomatal conductance and transpiration rate were not affected until 3 weeks after GLK-8924 treatment. With 24 g NutriCote per pot, lamina N concentration in GLK-8924 treated plants was 12.5-fold of that in untreated plants, regardless of GLK-8924 concentration. Lamina P, K, Fe, and Cu were greater while S, Ca, Mg, Mn, B, and Zn were not affected by GLK-8924. The reduced growth by GLK-8924 may be due to the reduced stomatal conductance while the increased growth by high fertilization may be due to influences on plant nutritional status.