The regeneration medium supplemented with 2.0 mg/L BAP and 0.1 mg/L IAA allowed high efficient shoot regeneration from leaf discs and petioles of Cichorium intybus L. var. sativus. Multiple shoots ranged from 10 to 14 per explant were observed only 10 to 15 days after the initial culture. Reduced nitrogen and sucrose levels influenced on shoot regeneration frequency and growth rates. Especially, in C. Intybus L. var. sativus cv. Cesare explants cultured in the medium containing 50 mg/L MS macroelement and 1.5% sucrose displayed high regeneration frequency of 100%.
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