Are the “Dentatun” Complex of Viburnum Species Genetically Distinct?

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  • 1 1Chicago Botanic Garden, Glencoe, IL 60022
  • | 2 2Horticulture Dept., Zoo Atlanta, Atlanta, GA 30315

Viburnum bracteatum Rehd. is a member of the “dentatum” complex represented by at least three types: V. bracteatum, V. dentatum L., and V. rafinesquianum Schult. V. bracteatum is an endangered species in Georgia and at the federal level is a candidate as an endangered species. Two populations were located in northwestern Georgia; however, there is some concern as to whether they are in fact V. bracteatum. To determine if it is possible to distinguish between the three Viburnum species, cellulose acetate electrophoresis to detect isozyme variation was done. Polymorphic enzymes resolved were alcohol dehydrogenase, malic dehydrogenase, glucose-6-phosphate dehydrogenase, malic enzyme, 6-phosphogluconate dehydrogenase, phosphoglucomutase, and phosphoglucose isomerase. Fresh bud tissue was used, and tissue samples were electrophoresed three times for each enzyme assayed. A review of 100 phylogeny trees created with Dollop analysis was done. V. rafinesquianum, the known sample of V. bracteatum, and the 12 samples of possible V. bracteatum were all equally parsimonious. V. dentatum was consistently an outgroup. In conclusion, isozyme variation can assist in Viburnum species distinction.

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