To investigate the differences of anatomical structure of neck tissue between bent-neck and strong-neck flowers, scanning electron microscopy of neck tissue during senescence of cut rose flowers held in deionized water or preservative solution (3% sucrose + 200 ppm HQS + 0.1 mM ethionine) was observed. Lignins in xylem, phloem, and interfascicular cambium of neck were stained to red by phloroglucine. More lignin was formed in the phloem of neck in rose flowers held in preservative solution than deionized water. Neck strength of cut rose could be increased by increase of lignin content, and this would prevent bent-neck and extend vase life. Parenchyma cells in neck part of rose flowers held in deionized water had thinner cell wall and less starch grains at senescence than those of flowers held in preservative solution at day 7. These starch grains would be used as energy source of rose flowers and extend vase life. Globular crystals were observed in the inner part of cells and had shape of large thorny. These crystals were cumulated in cell walls, then would prevent the activity of cell wall decomposition or increase cell wall permeability.