Induction of In Vitro Cultured Masses of Shoot Primordia of Hybrid Statice and Its Cryopreservation by Vitrification

in HortScience
View More View Less
  • 1 Shimane Agricultural Experiment Station, Ashiwata 2440, Izumo, Shimane, 693, Japan
  • | 2 Yokota Town Office, Yokota, Nita, Shimane, 699-18, Japan
  • | 3 Asabucho 1-5-23, Kitaku, Sapporo, 001, Japan

Bulbous structures consisting of meristematic clumps (designated “shoot primordia”) were induced from a meristematic culture of a hybrid statice (Limonium altaica Mill. × L. caspium Mill., cv. Blue Symphonet). The shoot tips were cultured in 25 mL of liquid 1/2 Murashige & Skoog (MS) medium supplemented with 0.44 μm BA and 0.054 μm NAA and 3% sucrose at pH 5.8 by vertically shaking at 2 rpm on rotating stages (1 m in diameter) at 25 °C. One month after inoculation of shoot tips, numerous small globular structures were formed and propagated vegetatively at a high rate following subculture. Segments of shoot primordia had developed into plantlets 2 weeks after transfer to solidified 1/2 MS medium supplemented with 0.44 μm BA and 1% sucrose. Plantlets successfully acclimated and grew into normal plants in a greenhouse. Cold-hardened, precultured small segments of shoot primordia were successfully cryopreserved in liquid N by vitrification. Vitrified and warmed segments plated on solidified 1/2 MS medium produced shoots about 21 d after plating. Cultured masses of shoot primordia appear promising for large-scale production and cryopreservation of annual and biennial statice. Chemical names used: 6-benzyladenine (BA); 1-naphthaleneacetic acid (NAA).

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 54 14 0
PDF Downloads 97 38 3