The hybrid origin of 23 rose (Rosa spp.) accessions was examined with three isozymes: acid phosphatase (E.C.220.127.116.11), malate dehydrogenase (E.C.18.104.22.168), and phosphoglucose isomerase (E.C.22.214.171.124). All three isozymes were useful for interspecific hybrid verification. This procedure was effective if the putative parents were known and differed in isozyme phenotype. To verify the origin of hybrid species or cultivars with hybrid origins, isozymes were useful but limited by the number of generations since the original hybridization and the number of accessions of the putative parental species assayed.
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