A Petiole Sap Nitrate Test for Cauliflower

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  • 1 Department of Soil, Water, and Environmental Science, University of Arizona, 429 Shantz, Building #38, Tucson, AZ 85721

This study was conducted to evaluate the accuracy of sap analysis using a portable nitrate ion meter for cauliflower (Brassica oleracea L. Botrytis Group, cv. Candid Charm) petiole nitrate determination. The relationship between NO3-N concentration in fresh petiole sap and in dried petiole tissue was studied for cauliflower grown in southern Arizona during the 1993–94 and 1994–95 growing seasons. Experiments were factorial combinations of three water rates and four N rates, both ranging from deficient to excessive. Petioles were collected throughout each season and were split for analysis of sap NO3-N and dried petiole NO3-N. Linear correlations between the two methods were similar in both seasons, with no consistent effect due to water application rate or crop maturity. Therefore, a single regression equation was derived: petiole sap NO3-N (mg·liter–1) = 0.047 × dry petiole NO3-N (mg·kg–1) + 218 (r2 = 0.772). This equation can be used to relate sap test measurements to existing guidelines for NO3-N concentrations in cauliflower petioles. These results suggest that the quick sap test, using the portable nitrate ion meter, is a valuable technique for monitoring N status of cauliflower.

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