Mature flowering Arbutus texana trees were successfully micropropagated from shoot tips. Optimum shoot proliferation was achieved on a basal medium consisting of WPM salts, MS vitamins, and sucrose supplemented with 11.1 or 22.2 μm BA and no auxin. Microcuttings rooted readily when pulsed with 6.1 μm IBA for 1 week and transferred to auxin-free medium. The addition of charcoal to the rooting medium improved root branching and elongation but suppressed root formation. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); indole-3-butyric acid (IBA).