Leaf Wounding Increases Efficiency of Agrobacterium-mediated Transformation of Apple

in HortScience
View More View Less
  • 1 Department of Plant Pathology, Cornell University, Geneva, NY 14456-0331

In vitro–grown leaves of Malus ×domestica Borkh. cv. Royal Gala were either crush-wounded with forceps, cut, or left whole, and then inoculated with A. tumefaciens strain EHA105 (p35SGUS_INT), with and without vacuum infiltration. Transformation was quantified 13 days after inoculation by determining the rate of β-glucuronidase (GUS) activity. Leaf wounding by crushing with nontraumatic forceps significantly increased transformation when compared with cutting leaves. Vacuum infiltration of inoculum had no effect on transformation.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 399 48 8
PDF Downloads 292 81 9