In poplar (Populus deltoides Bartr.ex Marsh), the development of bud dormancy is initiated by short-day (SD) photoperiods. The degree of bud dormancy, expressed as days to budbreak, increased from ≈10 days for plants grown under long-days to >200 days after 10 weeks of SD exposure. We investigated quantitative and qualitative changes in protein fractions extracted from terminal buds, lateral buds, bark, and leaves of poplar plants during the induction of bud dormancy by 2-D PAGE. While total protein contents(as milligrams per gram fresh weight) in leaves, terminal, and lateral buds did not change significantly during SD treatment, bark protein content increased about five-fold in 10 weeks. The results of 2-D PAGE analysis indicated that there was a significant change in protein profiles in terminal and lateral buds, leaves, and bark. The results suggested that SD treatment in poplar plants causes substantial changes in protein profiles during the induction of bud dormancy.
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