Peroxidases are an enzyme family that displays a wide range of forms, functions, and distribution in the plant kingdom. Peroxidase extracted from horseradish is one of a few enzymes that had been widely used in industrial and clinical research. Horseradish peroxidase (HRP, EC 184.108.40.206, donor: hydrogen-peroxide oxidoreductase) has been used to estimate the levels of other enzymes, in immunoassay, bio-bleaching processes, and in lignin degradation for fuel production. Despite its extensive use, little is known about HRP distribution in horseradish plants. Four commercial horseradish cultivars (IL-647, IL-1069, IL-1573, and IL-1590) were evaluated for HRP activity in the main root, lateral roots, leaves, and leaf blades. HRP activity was highest in the main root followed by lateral roots. However, only a trace amount of HRP activity was detected in leaf blade and leaf petiole. IL-1573 has the highest HRP activity, while IL-647 has the lowest activity. Site of HRP synthesis and its distribution during horseradish plant growth and development will be described.
If the inline PDF is not rendering correctly, you can download the PDF file here.