Rubus idaeus L. cv. Heritage raspberries were placed in controlled environment chambers (25°C, 14-hour photoperiod, 2.0 kPa vapor pressure deficit, CO2 concentration of 380 mol·m-2·s-1) to study the effects of drought stress on leaf gas exchange and stem water potential. Whole-plant photosynthesis (Pn) and transpiration were sensitive to drought stress and gradually decreased from the second day of the study until rehydration. Stomatal aperture feed-back regulation was present during the initial 48 hours of the study with transpiration rates dropping in response to a decrease in stem water potential. Spatial differences were also present with leaf Pn, and stomatal and CO2 conductance values of the younger, distal (i.e., closer to the apex) leaves decreasing at a faster rate than the older, proximal leaves (i.e., close to crown). Evidence of increased mesophyll resistance to drought stress was apparent with ci either remaining constant or increasing, while Pn and carboxylation efficiency simultaneously decreased. Protection of the underlying photochemistry was evident with parahelionastic leaf movements which resulted in a reduction in the effective leaf area and subsequent heat load. Therefore, an optimum balance between water loss and ci existed, and an alteration in these rates represented a stomatal conductance adjustment to match the intrinsic photosynthetic capacity rather than just a causal relationship.