Direct Shoot Organogenesis of Medicago sativa

in HortScience
Authors:
Guochen YangDept. of Natural Resources & Environmental Design, North Carolina A&T State Univ., Greensboro, NC 27411

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Marihelen Kamp-GlassDept. of Natural Resources & Environmental Design, North Carolina A&T State Univ., Greensboro, NC 27411

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An efficient and reliable protocol of in vitro shoot regeneration must be first established to have a successful genetic transformation. As a member of legume family, alfalfa is very difficult for direct shoot regeneration. There is no published information on direct shoot organogenesis, although success has been well documented on embryogenesis, which must go through callus stage. Different plant growth regulators at various concentrations were evaluated for callus initiation, development, and direct shoot regeneration. Multiple shoots were produced directly from each individual explant. This will provide an efficient means for production of transgenic alfalfa plants. Therefore, genetic transformation of Medicago germplasm will be significantly expedited.

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