In Vivo and in Vitro Propagation of Selected Cabbage (Brassica oleracea L. var. capitata) Clones

in HortScience
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  • 1 Dept. de Agricultura, Univ. Federal de Lavras, Cx. Postal 37, CEP 37200-000 Lavras-MG-Brazil

Hybrid cabbage cultivars can be produced via seed-propagated self-incompatible (SI) inbred lines, or, alternatively, via vegetative propagation of SI clones. Cabbage clones differ in their ability to undergo vegetative propagation, a fact that appears to be related to the degree of differentiation of the axillary buds inside the head. A procedure for in vivo and in vitro propagation is described for cabbage clones known for difficulty in undergoing vegetative propagation. Cuttings from clonal families 800 (easy-to-propagate) and 007 (difficult to propagate) were immersed in indolebutyric acid (IBA—0, 5, 25, and 125 mg·L–1) + boric acid (100 mg·L–1) + sucrose (20 g·L–1) for 15 hours and maintained in glasshouses. Induction of roots was more effective with 125 mg·L–1 IBA supplemented with boric acid and sucrose. This treatment showed the highest frequency of rooting and the largest number of roots per cutting. The in vitro system of propagation was performed on the basal medium of Murashige and Skoog (MS), to which triadizuron (TDZ), benzyladeninepurine (BAP), and kinetin (Kin) were added in different combinations. TDZ was more effective than BAP or Kin in the promotion of shoot regeneration.

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