Molecular Mapping of Capsicum and the Comparisons of Synteny with Tomato

in HortScience
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  • 1 Dept. of Plant Breeding and Biometry, Cornell Univ., Ithaca, NY 14853

Pepper (Capsicum spp.) has the same basic chromosome number as tomato and other solanaceous plant species (2n = 2x = 24). By using the probes mostly from a tomato map, we have generated three molecular maps of pepper from interspecific F2 crosses of C. frutescens BG 2814-6, C. chinense PI 159234 and C. annuum `NuMex RNaky' with restriction fragment length polymorphisms, isozymes, random amplified polymorphic DNAs, and morphological traits. The best developed map is from C. annuum × C. chinense F2 cross, which currently has 366 markers covered 1081 cM in 18 linkage groups. Three linkage groups were assigned to three chromosomes based on primary trisomics. Several disease resistance genes including monogenic resistance to potyviruses and quantitative trait loci for resistance to tobacco mosaic virus and cucumber mosaic virus have been mapped. The distribution of allele frequency and marker segregation ratios have been analyzed. Chi-square analyses of all clones showed more skewing of segregation ratios in C. annuum × C. chinense population than the other two populations. The skewing occurs throughout the genome and tends towards heterozygote and one of the parents. The order of markers among three pepper maps will be compared and the comparisons of synteny between pepper and tomato maps will be described. A composite of three pepper maps will be presented using JoinMap software.

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