Tree-ripe `Tommy Atkins' mangoes were not injured during storage in controlled atmospheres (CA) for 21 days at 8°C, and the fruit resumed ripening after transfer to air at 20°C (Bender et al., 1995). In our study, tree-ripe `Keitt' mangoes were stored at 5 and 8°C in either 10% or 25% CO2 combined with 5% O2 with control fruit maintained in air. Control fruit had higher percentages of electrolyte leakage than CO2-treated fruit at transfer from the CA and after 3 days in air at 20°C. Fruit stored in 25% CO2 at 5°C had significantly higher concentrations of 1-aminocyclopropane-1-carboxylic acid (ACC), over 0.5 nmol ACC/g fresh weight in mesocarp tissue. All the other treatments had similar ACC levels (<0.3 nmol/g fresh weight) after 21 days in CA. Ethylene production rates at both temperatures were significantly lower in the 10% CO2 treatment than in control fruit and were not detectable in 25% CO2. Ethylene production was similar in all treatments after transfer to air. Fruit from the 25% CO2 treatment at 5°C developed dull, green-grayish spots on the epidermis, but otherwise epidermal color, as determined by chroma and hue angles, did not differ among the treatments. There also were no differences in flesh color and flesh firmness.
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