Evaluation of Stomatal Density and Calcium Concentration on Pods of Six Commercial Cultivars of Snap Beans (Phaseolus vulgaris L.) at Four Planting Dates

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  • 1 Dept. of Horticulture, Univ. of Wisconsin–Madison, Madison WI 53706

Pod stomatal density and Ca concentration levels were analyzed for six commercial snap bean cultivars harvested at four planting dates in an attempt to find morphological traits that are related to cultivar differences in pod Ca concentration. The experimental layout was a randomized complete-block design with two replications per planting date, all grown in one location. Snap beans were planted at 1 week intervals beginning 9 June 9 1995 and were harvested in August. Sampling consisted of five pod sizes (1, 2, 3, 4, and 5 according to commercial standards) from each genotype. Stomatal countings were performed using a microscope linked to a television camera. Determinations for pod Ca concentration were made using an atomic absorption spectrophotometer. No differences were detected for pod Ca concentration among planting dates, although there were differences for pod Ca concentration and stomata density among cultivars. Pod stomatal density was positively correlated to pod Ca concentration (R2 = 0.60), while pod maturity appeared to be negatively correlated to pod Ca concentration (R2 = 0.37) and pod stomatal density (R2 = 0.49).

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