Shoot proliferation of Gardenia jasminoides was achieved from cultured shoot tips on Nitsch and Nitsch medium supplemented with different levels (0.0–0.6 mg·L–1) of zeatin, BAP, BA, TDZ, and kinetin. Zeatin proved to be the most effective cytokinin for stimulating shoot proliferation. Shoot length obtained with zeatin was shorter than with other cytokinins and shoot leaves were narrower. Shoot tips were cultured on Nitsch and Nitsch medium supplemented with BA at 4.0 mg·L–1 combined with IAA at 0.0–0.2 mg·L–1. The results indicated that BA at 4.0 mg·L–1 with 0.1 IAA produced greater shoot proliferation. Plantlets regenerated in vitro were then transferred to a mixture of 1 peat: 1 perlite: 1 soil and acclimatized for potting. Our results show that micropropagation of Gardenia has high potential for use in commercial industry.