Early Nodulin Gene (ENOD2) Expression in Maackia amurensis Rupr. & Maxim. (Amur maackia)

in HortScience
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  • 1 1Interdepartmental Plant Physiology Program, Iowa State Univ., Ames, IA 50011-0011
  • | 2 2Dept. of Horticulture, Iowa State Univ., Ames, IA 50011-0011
  • | 3 3Dept. of Botany, Iowa State Univ., Ames, IA 50011-0011

A complete picture of legume nodulation has yet to be elucidated. Most studies of the molecular mechanisms responsible for nodule organogenesis have focused on herbaceous legumes. We investigated the presence of a putative ENOD2 gene and studied the temporal and organ-specific production of its transcripts in an ornamental woody legume, Amur maackia. Primers derived from proline-rich pentapeptide repeats of conserved ENOD2 sequences and the genomic DNA of Amur maackia were used to obtain a 543-bp PCR fragment. Southern and Northern blots were probed with this cloned fragment. The Amur maackia genome contained an ENOD2 sequence that is similar to sequences in other species. Expression of the putative ENOD2 gene was detected in roots, 4 days after rhizobial inoculation, but not in leaves or stems. New data on the characteristics of nodulin genes in woody legumes will be beneficial in clarifying the nature and evolution of nodulation in legumes and may have implications for developing sustainable nursery production protocols.

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