Establishment of Aloe, Gasteria, and Haworthia Shoot Cultures from Inflorescence Explants

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  • 1 The Holden Arboretum, 9500 Sperry Road, Mentor, OH 44060
  • | 2 Department of Plant Sciences, 303 Forbes Building, University of Arizona, Tucson, AZ 85721

Shoot cultures of Aloe, Gasteria, and Haworthia species were initiated directly from immature inflorescences. Explants placed on a modified MS medium containing 5.4 mm zeatin riboside initiated shoots within 8 to 12 weeks. Long-term shoot cultures were established and maintained on media containing either 5.4 μm zeatin riboside or 4 μm BA. Shoots easily rooted in vitro, and rooted plantlets were esablished in soil. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); 6-[4-hydroxy-3-methyl-but-2-enylamino]purine riboside (zeatin riboside).

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