Cotton Fiber as a Substitute for Agar Support in Tissue Culture

in HortScience
View More View Less
  • 1 Research Institute of Pharmaceutical Sciences, The University of Mississippi, MS 38677
  • | 2 Department of Plant and Soil Sciences, Mississippi State University, Mississippi State, MS 39762
  • | 3 Research Institute of Pharmaceutical Sciences, The University of Mississippi, MS 38677
  • | 4 Setor de Biotecnologia Vegetal—Universidade de Ribeirao Preto, Ribeirao Preto, SP, Brazil

Cotton fibers were tested as a substitute for agar in tissue culture. The cost of agar has prompted us to search for an alternative more economical medium support. Effectiveness as a medium support was evaluated in terms of callus maintenance and shoot organogenesis using Artemisia, Agrostis, and Taxus. Taxus and Agrostis calli cultivated on liquid media with cotton fiber as medium support (25 ml of medium per gram of cotton) grew better than calli on agar (0.8% w/v). There were no significant differences in shoot organogenesis of Artemisia and Agrostis grown in 25 ml of medium per gram of cotton from those grown in agar medium.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 197 38 3
PDF Downloads 199 70 7