Citrus macrophylla is an important citrus rootstock for Mexican lemon (Citrus aurantifolia S.). Citrus are highly dependent of vesicular–arbuscular mycorrhizal (VAM) fungi. Four Glomus species were screened for their symbiotic response with C. macrophylla. Seedlings were inoculated with VAM fungi in pots containing sterilized soil. After 3 and 4 months, plants were harvested. Glomus fasciculatum (following by G. intraradices) gave the greatest improvements in growth, resulting in larger plant height and higher shoot dry weight. Glomus aggregatum, G. mosseae, and control plants showed the lowest rates of growth. Plants inoculated with the first three species showed the highest percent of root length colonized. However G. aggregatum gave the highest values of visual density of endophyte in root and soil hyphae. Root colonization and soil hyphae were lowest in plants with G. mosseae.