Interaction between irradiance levels (5–40 mM–m–2–s–1) and iron chelate sources (FeNa2EDTA and FeNaDTPA) on the establishment, growth, and proliferation of shoot tips of Carica papaya were tested. Reduced irradiance level (5 mM–m–2–s–1) enhanced the establishment of shoot tips regardless of the source of iron chelate tested. At higher irradiance levels (30 and 40 mM–m–2–s–1), presence of FeNaDTPA in the medium enhanced establishment of shoot tips. Continuous or alternating light/dark (16/8 h) photoperiods at high irradiance levels had no effect on the establishment or growth of the culture. At higher irradiance levels, the cultures produced smaller leaves as compared to lower irradiance levels. Low irradiance and FeNa2EDTA was preferred during the proliferation stage.
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