The Mass Production and Acclimatization of Somatic Embryos in Oenanthe stolonifera DC.

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  • 1 Dept. of Horticulture, Seoul National Univ., Korea

Immature florets were inoculated on MS basal salt media (3% sucrose + 0.8% agar) + 1.0 mg 2,4-D/liter to induce embryogenic callus. Induced embryogenic callus was sieved through serial metal mesh screens (40 and 60 mesh) and placed on the embryo induction media at different concentrations of cytokinins and osmotica. BAP (0.1 mgliter–1) or TDZ (0.001, 0.01, 0.1, and 1.0 mgliter–1) were treated on 1/2 MS (3% sucrose) according to the following schedules: 1) first 2 weeks of treatments, followed by 3 weeks of 1/2 MS (3% sucrose) (FT); 2) first 3 weeks of 1/2 MS (3% sucrose), followed by 2 weeks of treatment (LT); and 3) 5 weeks of treatments. PEG, mannitol (0, 2.5%, 5.0%, 7.5%, and 10.0%), and sucrose (0%, 3%, 6%, 9%, and 15%) also were used as osmotica with ABA (0.05 mgliter–1), respectively. The treatment schedules were FT and LT. Several good quality embryos were produced at 0.001 mg TDZ/liter, 0.1 mg BAP/liter, or 7.5% and 10.0% PEG (only FT). Abnormal embryos were significantly reduced at 7.5% and 10.0% PEG. Leaves of the plants obtained through somatic embryos were compared with those of seed-propagated plants before and after acclimatization using SEM. Epidermal and conductive tissues developed little in the plants before acclimatization; however, they developed gradually after acclimatization and were similar to those of seed-propagated plants.

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