Variation of Chromosome Numbers in Easter Lily Regenerated from Ovary Tissues

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  • 1 1Dept. of Botany, North Dakota State Univ., Fargo, ND 58105
  • | 2 2Dept. of Plant Sciences, North Dakota State Univ., Fargo, ND 58105

Easter lily (Lilium longiflorum L.) cultivars Ace and Nellie White were regenerated through the culture of immature ovary tissues. Shoot initiation and proliferation were most efficient when a modified Murashige and Skoog (MS) medium containing 5% sucrose, 1 mg 2,4-D/liter, and 2 mg benzylamino purine (BAP)/liter was used. The shoots, when divided and subcultured on the same medium, formed roots within 4 weeks. The rooted plants were transferred to soil in a greenhouse. Root-tip smears made from the regenerated plants showed a range of variation in chromosome numbers from 10 to 25 per cell, in contrast to the bulb-grown plants, which had 2x = 24 chromosomes per cell. The mixoploid condition existed in many regenerants exhibiting chromosome number variation in different root cells of the same plant.

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