Nucleotide Pools in Imbibing Tomato Seeds

in HortScience
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  • 1 School of Horticulture, Univ. of Western Sydney, Richmond, NSW, Australia NSW 2753.

HPLC was used to measure nucleotide pools in tomato seeds during the 3 h following imbibition in water. In dry seeds, nucleotides were predominantly in the form of monophosphates. During the first 2 h post-imbibition, the monophosphate levels declined and there were sharp increases in the amounts of diphosphates, followed by triphosphates. Between the 2nd and 3rd h, the di- and triphosphate levels continued to increase and the monophosphate levels began to recover, especially in the case of UMP—presumably the result of degradation of unwanted mRNAs left over from the maturation phase of seed development. The adenylate energy charge increased during imbibition and, within 3 h, reached a value close to that of normal active tissue.

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