An Ipomoea accession from Indonesia, originally classified as I. trifida, was found to segregate in flower morphology. It was hypothesized to be either a very close relative of I. batatas (6x sweetpotato), or a hybrid between I. batatas (6x) and I. trifida (2x). Twelve seedlings of this accession were grown and precise measurements of sepal angle, corolla shape, and root morphology were taken. Samples were also compared on the DNA level using molecular markers. Based on morphological measurements, it was found that some individual seedlings of the unknown Ipomoea accession were not significantly different than I. batatas; others were not significantly different than I. trifida. The control I. batatas and I. trifida lines were significantly different from each other. DNA flow cytometry was used to determine that all seedlings were diploids with the same amount of DNA per cell. Overall plant morphology and molecular analysis confirmed that all of the seedlings were very closely related and the segregation in flower morphology was not due to a seed mixture. This data is consistent with the hypothesis that the accession is a hybrid between I. batatas and I. trifida.