Molecular Markers and Linkage to the Sw-5 Locus for Resistance to Tomato Spotted Wilt Virus (TSWV)

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  • 1 1Agronomy & Horticulture Dept., Brigham Young Univ., Provo UT 84602
  • | 2 2Gulf Coast Research & Education Center, IFAS, Univ. of Florida, 60th St. E., Bradenton, FL 34203
  • | 3 3Dept. of Biological Sciences, Univ. of Arkansas, Fayetteville, AR 72701.

Sw-5 is a locus introgressed from Lycopersicon peruvianum to some L. esculentum lines conferring dominant resistance to TSWV. Restriction fragment length polymorphism (RFLP) analyses positions Sw-5 to the long arm of chromosome 9 in the sub-telomeric region between CT71 and CT220. RFLP analyses suggest the introgressed region begins distal to CT71, includes CT220, and may extend to the telomere. Randomly amplified polymorphic DNA (RAPD) analyses with >700 random 10-mer primers identified a single 2.2-kbp band with one primer (primer #72 GAGCACGGGA) that is tightly linked to Sw-5. However, we have also produced an equivalent 2.2-kbp band in analysis of other TSWV-susceptible tomato breeding lines. Thus, this band likely derives from L. esculentum DNA very near to Sw-5 and the introgressed region. Additional analyses have recently detected a potential co-dominant RAPD polymorphism linked to Sw-5.

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